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Registro Completo |
Biblioteca(s): |
Embrapa Suínos e Aves. |
Data corrente: |
25/02/2011 |
Data da última atualização: |
25/02/2011 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
BACKES, C.; GUERRINI, I. A.; OLIVEIRA, M. R. de; MIGGIOLARO, A. E.; CORREA, J. C.; BÜLL, L. T. |
Afiliação: |
CLARICE BACKES, CNPQ/UNESP; IRAÊ AMARAL GUERRINI, CNPq; MAURÍCIO ROBERTO DE OLIVEIRA, UNESP; ALESSANDRA ELENA MIGGIOLARO, UNESP; JULIANO CORULLI CORREA, CNPSA; LEONARDO THEODORO BÜLL, UNESP. |
Título: |
Dinâmica do nitrogênio em função da aplicação de lodos de esgoto em sistema de plantio direto. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa, MG: SBCS, 2010. 1 CD-ROM. FERTBIO 2010. |
Idioma: |
Português |
Notas: |
Projeto/Plano de Ação: 01.09.10103-04. |
Thesagro: |
Lixiviação; Mineralização; Solo. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/28688/1/juliano-dinamica.pdf
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Marc: |
LEADER 00942nam a2200205 a 4500 001 1879096 005 2011-02-25 008 2010 bl uuuu u00u1 u #d 100 1 $aBACKES, C. 245 $aDinâmica do nitrogênio em função da aplicação de lodos de esgoto em sistema de plantio direto. 260 $aIn: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 29.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 13.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 11.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 8., 2010, Guarapari. Fontes de nutrientes e produção agrícola: modelando o futuro: anais. Viçosa, MG: SBCS, 2010. 1 CD-ROM. FERTBIO 2010.$c2010 500 $aProjeto/Plano de Ação: 01.09.10103-04. 650 $aLixiviação 650 $aMineralização 650 $aSolo 700 1 $aGUERRINI, I. A. 700 1 $aOLIVEIRA, M. R. de 700 1 $aMIGGIOLARO, A. E. 700 1 $aCORREA, J. C. 700 1 $aBÜLL, L. T.
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Embrapa Suínos e Aves (CNPSA) |
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Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental; Embrapa Pecuária Sudeste. |
Data corrente: |
04/09/2017 |
Data da última atualização: |
16/01/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
BRITO, L. G.; NERY, L. de O.; BARBIERI, F. da S.; HUACCA, M. E. F.; PEREIRA, S. dos S.; SILVA, R. R. da; FERNANDES, C. F. C.; OLIVEIRA, M. C. de S. |
Afiliação: |
LUCIANA GATTO BRITO, CPATU; Louí de Oliveira Néry, Bolsista CPATU; FABIO DA SILVA BARBIERI, CPATU; Maribel Elizabeth Funes Huacca, UNIR; Soraya dos Santos Pereira, Fiocruz Rondônia; RENATA REIS DA SILVA, CPATU; Carla Freire Celedonio Fernandes, Fiocruz Rondônia; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
Título: |
Molecular quantitative assay for esterase-mediated organophosphate resistance in Rhipicephalus microplus. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Ticks and Tick-borne Diseases, v. 8, n. 5, p. 725-732, 2017. |
DOI: |
https://doi.org/10.1016/j.ttbdis.2017.05.006 |
Idioma: |
Inglês |
Conteúdo: |
The use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and the other OP-resistant (LC50 = 8.14 μg/cm2). To promote the OP enzyme detoxification, groups of 10 females of the resistant strain were immersed in solutions of diazinon in technical grade at concentrations of 1.0 mg/ml, 2.5 mg/ml, and 5.0 mg/ml. The ticks that survived diazinon exposure were submitted to qPCR assay, which enabled observing an increase in AChE2 and EST1 synthesis in the OP-resistant strain when compared to the susceptible strain. The initial results of expression analysis suggest that the qPCR assay can discriminate OP-resistant and susceptible populations. The development and improvement of molecular diagnostic tests to identify pesticide resistant R. microplus populations are priorities and in the near future it will be important to expand the molecular targets involved in OP resistance, which could be used for better selection of effective strategies to control cattle tick populations. MenosThe use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and the other OP-resistant (LC50 = 8.14 μg/cm2). To promote the OP enzyme detoxification, groups of 10 females of the resistant strain were immersed in solutions of diazinon in technical grade at concentrations of 1.0 mg/ml, 2.5 mg/ml, and 5.0 mg/ml. The ticks that survived diazinon exposure were submitted to qPCR assay, which enabled observing an increase in AChE2 and EST1 synthesis in the OP-resistant strain when compared to the susceptible strain. The initial results of expression analysis suggest that the qPCR assay can discriminate OP-resistant and susceptible populations. The development and improvement of molecular diagnostic tests to identify pesticide resistant R. microplus populations are priorities and in the near future ... Mostrar Tudo |
Palavras-Chave: |
Cattle tick; Organophosphate resistance. |
Thesaurus NAL: |
esterases. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02493naa a2200253 a 4500 001 2076221 005 2019-01-16 008 2017 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.ttbdis.2017.05.006$2DOI 100 1 $aBRITO, L. G. 245 $aMolecular quantitative assay for esterase-mediated organophosphate resistance in Rhipicephalus microplus.$h[electronic resource] 260 $c2017 520 $aThe use of pesticides is the main tool to control infestations of the cattle tick Rhipicephalus microplus, and organophosphate (OP) is one of the most used compounds for this purpose. Carboxylesterases (ChEs) are targets for OP pesticides in arthropods, and acetylcholinesterase 2 (AChE2) and esterase 1 (EST1) are metabolic enzymes involved in the xenobiotic detoxification process. The increase in the synthesis of these enzymes can be detected by the quantitative polymerase chain reaction (qPCR) assay, which was used to identify cattle tick populations resistant to OP pesticides. For that, two field populations of R. microplus were used, one previously identified by the larval packet test (LPT) as OP −sensitive (LC50 = 0.13 μg/cm2) and the other OP-resistant (LC50 = 8.14 μg/cm2). To promote the OP enzyme detoxification, groups of 10 females of the resistant strain were immersed in solutions of diazinon in technical grade at concentrations of 1.0 mg/ml, 2.5 mg/ml, and 5.0 mg/ml. The ticks that survived diazinon exposure were submitted to qPCR assay, which enabled observing an increase in AChE2 and EST1 synthesis in the OP-resistant strain when compared to the susceptible strain. The initial results of expression analysis suggest that the qPCR assay can discriminate OP-resistant and susceptible populations. The development and improvement of molecular diagnostic tests to identify pesticide resistant R. microplus populations are priorities and in the near future it will be important to expand the molecular targets involved in OP resistance, which could be used for better selection of effective strategies to control cattle tick populations. 650 $aesterases 653 $aCattle tick 653 $aOrganophosphate resistance 700 1 $aNERY, L. de O. 700 1 $aBARBIERI, F. da S. 700 1 $aHUACCA, M. E. F. 700 1 $aPEREIRA, S. dos S. 700 1 $aSILVA, R. R. da 700 1 $aFERNANDES, C. F. C. 700 1 $aOLIVEIRA, M. C. de S. 773 $tTicks and Tick-borne Diseases$gv. 8, n. 5, p. 725-732, 2017.
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